La determine 12 représente l’observation microscopique réalisée en microscopie optique, à l’aide d’un microscope Leica® style Orthoplan, d’une coupe transversale d’un expiant cutané à J5 après traitement par P1 (P1J5), en paraffine colorées au trichrome de Masson variante de Goldner. La peau qui vieillit perd son élasticité et
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